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METHODS

 Yeast Analysis
 
  3.1Yeast Mass Determination
  3.1.1Yeast Cell Count
  3.1.1.1Haemocytometry
  3.1.1.2Electronic Counter
  3.1.1.3Photometric Determination
  3.1.1.4Count and Viability of Yeast Cells with a Flow Cytometer
  3.1.2Yeast Mass
  3.1.2.1Centrifugation
  3.1.2.2Dry Weight
  3.2Yeast Viability
  3.2.1Staining Methodes
  3.2.1.1Methylene Blue/Violet Stain
  3.2.1.2Fluorescence Stain
  3.2.2Growth Techniques
  3.2.2.1Slide Culture Techniques
  3.3Yeast Morphology
  3.3.1Microscopic Analysis
  3.3.1.1Cell Morphology
  3.3.2Cell Colony Morphology
  3.3.2.1Yeast Giant Colonies
  3.3.2.2Morphology on WLN Agar
  3.4Yeast Strain Storage, Supply and Propagation
  3.4.1Yeast Storage at Ultra-Low Temperatures
  3.4.2Yeast subculturing for short-term storage
  3.4.3Yeast strain transport
  3.4.4Yeast Propagation (Laboratory Stages)
  3.5Yeast Characterisation
  3.5.1Detection and Enumeration of Respiratory Deficient Mutants
  3.5.2Yeast Characterisation by Fermentation in EBC Tall Tubes
  3.5.3Floculation
  3.5.3.1Gilliland Method
  3.5.3.2Hough Method
  3.5.3.3Helm Method